Journal: ACS Omega

Article Title: Engineering Delivery of Nonbiologics Using Poly(lactic- co -glycolic acid) Nanoparticles for Repair of Disrupted Brain Endothelium

doi: 10.1021/acsomega.0c01517

Figure Lengend Snippet: Internalization and targeting efficiency studies of conjugated and unconjugated PLGA NPs. (A–D) Fluorescence images (green) showing the specificity of PSGL-1 conjugated PLGA NPs to either inactivated or TNF-α activated BECs. (E) Quantitative analysis of the specificity of PSGL-1-conjugated PLGA NPs. Data represent mean ± SD of three to six independent experiments. * p < 0.05 compared to the solid black bar. (F–H) Targeting efficiency study of conjugated PLGA NPs to endothelial cells exposed to mechanical trauma. (F) Control cells incubated with PSGL-1-conjugated PLGA NPs for 1 h. (G) Endothelial cells exposed to mechanical trauma and then incubated with PSGL-1-conjugated PLGA NPs for 1 h. The area of detached cell is apparent and indicated by a dotted line for better illustration. (H) Quantitative analysis of the specificity of PSGL-1-conjugated PLGA NPs. Data represent mean ± SD of three to six independent experiments. * p < 0.05.

Article Snippet: Eighty microliters of 500 μg/mL mouse PSGL-1 antibody (Sino Biological, 50770-MCCH) was added to the NP solution and incubated for 24 h at 4 °C.

Techniques: Fluorescence, Incubation